General description

Fructose is a monosaccharide naturally found in honey and fruits. Several studies have linked fructose to the development of the metabolic syndrome particularly through promoting insulin resistance in the liver. It has also been implicated in the obesity epidemic. Studies in animals have revealed high fructose intake during pregnancy and lactation may lead to metabolic dysfunctions in the mother and the newborn. Fructose measurements can provide useful insights into metabolic and biochemical functions.Fructose concentration is determined by a coupled enzyme assay, which results in a fluorometric (lex = 535/lem = 587 nm) product proportional to the fructose present.

Principle

Fructose concentration is determined by a coupled enzyme assay, which results in a fluorometric (λ_ex = 535/λ_em = 587 nm) product proportional to the fructose present.

Suitability

This kit is a highly sensitive assay for determining D-fructose levels (ranging from 20-100 pmole/well) in a variety of biological samples.